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C. elegans plasmid for expressing nematode codon-optimized Cas9 together with a single guide RNA (sgRNA).Sequence Author: Goldstein Lab / Addgene #47549 SV40 NLS pDD162 (Peft-3::Cas9 + Empty sgRNA) 8113 bp Cas92241 .. 6497 = 4257 bp1368 amino acids = 158.4 kDa4 segments = 4104 bpProduct: S. pyogenes Cas9, codon optimized for C. elegans with synthetic intronsCas92241 .. 6497 = 4257 bp1368 amino acids = 158.4 kDa4 segments = 4104 bpProduct: S. pyogenes Cas9, codon optimized for C. elegans with synthetic intronsCas92241 .. 6497 = 4257 bp1368 amino acids = 158.4 kDa4 segments = 4104 bpProduct: S. pyogenes Cas9, codon optimized for C. elegans with synthetic intronsCas92241 .. 6497 = 4257 bp1368 amino acids = 158.4 kDa4 segments = 4104 bp Segment 1: 2241 .. 3284 = 1044 bp 348 amino acids = 39.8 kDaProduct: S. pyogenes Cas9, codon optimized for C. elegans with synthetic intronsCas92241 .. 6497 = 4257 bp1368 amino acids = 158.4 kDa4 segments = 4104 bp Segment 2: 3336 .. 4406 = 1071 bp 357 amino acids = 42.2 kDaProduct: S. pyogenes Cas9, codon optimized for C. elegans with synthetic intronsCas92241 .. 6497 = 4257 bp1368 amino acids = 158.4 kDa4 segments = 4104 bp Segment 3: 4458 .. 5483 = 1026 bp 342 amino acids = 39.9 kDaProduct: S. pyogenes Cas9, codon optimized for C. elegans with synthetic intronsCas92241 .. 6497 = 4257 bp1368 amino acids = 158.4 kDa4 segments = 4104 bp Segment 4: 5535 .. 6497 = 963 bp 321 amino acids = 36.6 kDaProduct: S. pyogenes Cas9, codon optimized for C. elegans with synthetic intronsCas92241 .. 6497 = 4257 bp1368 amino acids = 158.4 kDa4 segments = 4104 bpProduct: S. pyogenes Cas9, codon optimized for C. elegans with synthetic intronsSV40 NLS6513 .. 6533 = 21 bp7 amino acids = 883.1 DaProduct: nuclear localization signal of SV40 large T antigenSV40 NLS6513 .. 6533 = 21 bp7 amino acids = 883.1 DaProduct: nuclear localization signal of SV40 large T antigenHA6534 .. 6560 = 27 bp9 amino acids = 1.1 kDaProduct: HA (human influenza hemagglutinin) epitope tagHA6534 .. 6560 = 27 bp9 amino acids = 1.1 kDaProduct: HA (human influenza hemagglutinin) epitope tagAmpR7916 .. 663 = 861 bp286 amino acids = 31.6 kDa2 segments Segment 1: signal sequence 7916 .. 7984 = 69 bp 23 amino acids = 2.6 kDaProduct: β-lactamaseconfers resistance to ampicillin, carbenicillin, and related antibioticsAmpR7916 .. 663 = 861 bp286 amino acids = 31.6 kDa2 segments Segment 2: 7985 .. 663 = 792 bp 263 amino acids = 28.9 kDaProduct: β-lactamaseconfers resistance to ampicillin, carbenicillin, and related antibioticsAmpR7916 .. 663 = 861 bp286 amino acids = 31.6 kDa2 segmentsProduct: β-lactamaseconfers resistance to ampicillin, carbenicillin, and related antibioticseft-3 promoter1625 .. 2224 = 600 bppromoter. Download Paint.NET .7888.-2 for Windows. Fast downloads of the latest free software! Click now.

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